Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz - Journal of Plant Diseases and Protection (2003) 110, p. 97 (Pospieszny et al.)

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H. Pospieszny, N. Borodynko and M. Palczewska (2003)
First record of Pepino mosaic virus in Poland
Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz - Journal of Plant Diseases and Protection 110 (1), 97-97
1st Conf. Intern. Working Groups on Legume and Vegtable Viruses, Bonn, Germany 4-9 Aug. 2002
Abstract: Since 1999, Pepino mosaic virus (PepMV) has been a new, very important pathogen infecting tomato (Lycopersicon esculentum) crops in Europe. Unexpectedly, within 2 years, the virus was found in several European countries and also in North America (VAN DER VLUGT et al. 2000; JORDÁ et al. 2001; FRENCH et al. 2001; MUMFORD and METCALFE 2001). PepMV can cause symptoms on both leaves and fruits, reducing yield and quality. In 2001, two tomato plants showing a yellow mosaic and leaf distortion were observed in a greenhouse where several varieties from different countries were tested. Examination of leaf sap using electron microscopy showed the presence of particles typical of potexviruses. Using polyclonal antibodies to PepMV (a kind gift of Plant Research International, Wageningen, the Netherlands) in ELISA, a positive reaction was obtained with sap of the plants tested. This identification was confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). Using primers derived from the RNA polymerase region of a UK isolate (MUMFORD and METCALFE 2001; FRENCH et al. 2001), RT-PCR gave the expected 312-bp product. for the Polish isolate. Under greenhouse conditions, the Polish isolate infected the following plant species: Nicotiana affinis, N. benthamiana, N. clevelandii, N. debneyi, N. glutinosa, N. tabacum, Nicandra physalodes, Solanum melongena and occasionally (probably during the warmer months) Physalis floridana. However, it did not infect Amaranthus retroflexus, Chenopodium amaranticolor, C. quinoa, Cucumis sativus, Petunia hybrida, Phaseolus vulgaris and Physalis floridana. Antiserum prepared against the Polish isolate of PepMV did not react with Potato virus X. Diagnostic techniques such as dot-blot immunoassay and IC/RT-PCR, using newly prepared antiserum, were studied. The usefulness of both techniques for PepMV diagnosis is presented.
Database assignments for author(s): Natasza Borodynko, Henryk Pospieszny

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy


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Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Pepino mosaic virus Tomato (Solanum lycopersicum) Poland