Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz - Journal of Plant Diseases and Protection (2000) 107, 548-552

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W. Menzel and E. Maiss (2000)
Detection of Chrysanthemum stunt viroid (CSVd) in cultivars of Argyranthemum frutescens by RT-PCR-ELISA
Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz - Journal of Plant Diseases and Protection 107 (5), 548-552
Abstract: A reverse transcription-polymerase chain reaction-enzyme-linked immunosorbent assay (RT-PCR-ELISA) for the detection of Chrysanthemum stunt viroid (CSVd) in Argyranthemum frutescens was developed. A sequence-specific, modified primer pair (5'-end labelled with digoxigenin or biotin) was used, defining a target sequence of 281 bp. The digoxigenin and biotin-labeled PCR products were directly bound to streptavidin-coated microtiter plates and detected using anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The described technique does not require denaturation and hybridization of a detection probe to the amplified products and operates without the use of hazardous Organic solvents and toxic or mutagenic stains. Only 6.5 h are required for the detection of CSVd from infected tissue. This ELISA technique could potentially replace common electrophoretic techniques for the detection of PCR products.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Wulf Menzel

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surveys/sampling/distribution


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Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Chrysanthemum stunt viroid Argyranthemum (crop)