Virus Genes (2002) 24, 231-234

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Yasuhiko Matsushita, Kuniaki Yoshioka, Takuma Shigyo, Hideki Takahashi and Hiroshi Nyunoya (2002)
Phosphorylation of the movement protein of Cucumber mosaic virus in transgenic tobacco plants
Virus Genes 24 (3), 231-234
Abstract: The 3a protein of Cucumber mosaic virus is essential for the cell-to-cell movement of the viral RNA through plasmodesmata. We have introduced an epitope peptide before the stop codon of the 3a protein and cloned the tagged ORF into a binary vector for Agrobacterium-mediated transformation. The established transgenic tobacco lines produced the 3a protein, which was specifically detected with anti-3a and anti-epitope antisera. Metabolic labeling and subsequent immunoprecipitation revealed that [32P]-orthophosphate was incorporated into the 3a protein. The phosphoamino acid analysis indicated that the 3a protein contained phosphoserine but not phosphothreonine or phosphotyrosine. This is the first demonstration of the 3a protein phosphorylation in planta.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Hideki Takahashi

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Cucumber mosaic virus Tobacco (Nicotiana tabacum)