The Plant Journal (2014) 78, 16-30

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Yanping Fu, Xiaoyuan Duan, Chunlei Tang, Xiaorui Li, Ralf T. Voegele, Xiaojie Wang, Guorong Wei and Zhensheng Kang (2014)
TaADF7, an actin-depolymerizing factor, contributes to wheat resistance against Puccinia striiformis f. sp. tritici
The Plant Journal 78 (1), 16-30
Abstract: The actin cytoskeleton is involved in plant defense responses; however, the role of the actin-depolymerizing factor (ADF) family, which regulates actin cytoskeletal dynamics, in plant disease resistance, is largely unknown. Here, we characterized a wheat (Triticum aestivum) ADF gene, TaADF7, with three copies located on chromosomes 1A, 1B, and 1D, respectively. All three copies encoded the same protein, although there were variations in 19 nucleotide positions in the open reading frame. Transcriptional expression of the three TaADF7 copies were all sharply elevated in response to avirulent Puccinia striiformis f. sp. tritici (Pst) infection, with similar expression patterns. TaADF7 regulated the actin cytoskeletal dynamics by targeting the actin cytoskeleton to execute actin binding/severing activities. When the TaADF7 copies were all silenced by virus-induced gene silencing, the growth of Pst hypha increased and sporadic urediniospores were observed, as compared with control plants, upon inoculation with avirulent Pst. In addition, the accumulation of reactive oxygen species (ROS) and the hypersensitive response (HR) were greatly weakened, whereas cytochalasin B partially rescued the HR in TaADF7 knock-down plants. Together, these findings suggest that TaADF7 is likely to contribute to wheat resistance against Pst infection by modulating the actin cytoskeletal dynamics to influence ROS accumulation and the HR.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Zhen Sheng Kang, Ralf T. Voegele

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Puccinia striiformis Wheat (Triticum)