Studies in Mycology (2006) 55, 147-161

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Gavin C. Hunter, Brenda D. Wingfield, Pedro W. Crous and Michael J. Wingfield (2006)
A multi-gene phylogeny for species of Mycosphaerella occurring on Eucalyptus leaves
Studies in Mycology 55, 147-161
Abstract: Species of the ascomycete genus Mycosphaerella are regarded as some of the most destructive leaf pathogens of a large number of economically important crop plants. Amongst these, approximately 60 Mycosphaerella spp. have been identified from various Eucalyptus spp. where they cause leaf diseases collectively known as Mycosphaerella Leaf Disease (MLD). Species concepts for this group of fungi remain confused, and hence their species identification is notoriously difficult. Thus, the introduction of DNA sequence comparisons has become the definitive characteristic used to distinguish species of Mycosphaerella. Sequences of the Internal Transcribed Spacer (ITS) region of the ribosomal RNA operon have most commonly been used to consider species boundaries in Mycosphaerella. However, sequences for this gene region do not always provide sufficient resolution for cryptic taxa. The aim of this study was, therefore, to use DNA sequences for three loci, ITS, Elongation factor 1-alpha (EF-1α) and Actin (ACT) to reconsider species boundaries for Mycosphaerella spp. from Eucalyptus. A further aim was to study the anamorph concepts and resolve the deeper nodes of Mycosphaerella, for which part of the Large Subunit (LSU) of the nuclear rRNA operon was sequenced. The ITS and EF-1α gene regions were found to be useful, but the ACT gene region did not provide species-level resolution in Mycosphaerella. A phylogeny of the combined DNA datasets showed that species of Mycosphaerella from Eucalyptus cluster in two distinct groups, which might ultimately represent discrete genera.
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Database assignments for author(s): Gavin C. Hunter, Pedro W. Crous, Brenda D. Wingfield, Michael J. Wingfield

Research topic(s) for pests/diseases/weeds:
identification/taxonomy


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Uwebraunia dekkeri Eucalypt (Eucalyptus) South Africa
Teratosphaeria cryptica Eucalypt (Eucalyptus)
Teratosphaeria nubilosa Eucalypt (Eucalyptus) South Africa
Phaeophleospora gregaria Eucalypt (Eucalyptus)
Pseudocercospora epispermogonia Eucalypt (Eucalyptus) South Africa
Neofusicoccum ribis
Teratosphaeria eucalypti Eucalypt (Eucalyptus) New Zealand
Suberoteratosphaeria suberosa Eucalypt (Eucalyptus) New Zealand
Pallidocercospora heimioides Eucalypt (Eucalyptus) Indonesia
Pallidocercospora irregulariramosa Eucalypt (Eucalyptus) South Africa
Parapallidocercospora colombiensis Eucalypt (Eucalyptus) Colombia
Uwebraunia commune Eucalypt (Eucalyptus) Spain (continental)
Amycosphaerella africana Eucalypt (Eucalyptus) South Africa
Mycosphaerella intermedia Eucalypt (Eucalyptus) New Zealand
Mycosphaerella madeirae Eucalypt (Eucalyptus) Portugal (Madeira)
Teratosphaeria mexicana Eucalypt (Eucalyptus)
Teratosphaeria molleriana Eucalypt (Eucalyptus) Portugal (continental)
Teratosphaeria molleriana Eucalypt (Eucalyptus) Australia (South+SE)
Passalora tasmaniensis Eucalypt (Eucalyptus) Australia (South+SE)
Austroafricana parva Eucalypt (Eucalyptus) South Africa
Austroafricana parva Eucalypt (Eucalyptus) Chile (continental)
Pallidocercospora heimii Eucalypt (Eucalyptus) Madagascar