Plant Disease (2016) 100, 1379-1387

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Rabson M. Mulenga, James P. Legg, Joseph Ndunguru, Douglas W. Miano, Eunice W. Mutitu, Patrick C. Chikoti and O.J. Alabi (2016)
Survey, molecular detection, and characterization of geminiviruses associated with cassava mosaic disease in Zambia
Plant Disease 100 (7), 1379-1387
Abstract: A survey was conducted from April to May 2014 in 214 farmers' fields located across six major cassava-producing provinces (Western, Northwestern, Northern, Luapula, Lusaka, and Eastern) of Zambia to determine the status of cassava mosaic disease (CMD) and the species diversity of associated cassava mosaic geminiviruses (CMG). Mean CMD incidence varied across all six provinces but was greatest in Lusaka Province (81%) and least in Northern Province (44%). Mean CMD severity varied slightly between provinces, ranging from 2.78 in Eastern Province to 3.00 in Northwestern Province. Polymerase chain reaction discrimination of 226 survey samples, coupled with complete DNA-A genome sequence analysis, revealed the presence of African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), and East African cassava mosaic Malawi virus (EACMMV) as single or mixed infections of different proportions. Single-virus infections were predominant, occurring in 62.8% (ACMV), 5.8% (EACMMV), and 2.2% (EACMV) of samples relative to mixed-virus infections, which occurred in 19.5% (ACMV + EACMMV), 0.4% (ACMV + EACMV), and 0.9% (ACMV + EACMV + EACMMV) of samples. Phylogenetic analysis revealed the segregation of virus isolates from Zambia into clades specific to ACMV, EACMV, and EACMMV, further confirming the presence of all three viruses in Zambia. The results point to a greater diversity of CMG across major cassava-growing provinces of Zambia and implicate contaminated cassava cuttings in disease spread.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Olufemi J. Alabi, James P. Legg, Douglas W. Miano

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


African cassava mosaic virus Cassava (Manihot esculenta) Zambia
East African cassava mosaic virus Cassava (Manihot esculenta) Zambia
East African cassava mosaic Malawi virus Cassava (Manihot esculenta) Zambia