Phytopathology (2009) 99, 711-715

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D. Pacifico, A. Alma, B. Bagnoli, X. Foissac, G. Pasquini, M. Tessitori and C. Marzachì (2009)
Characterization of bois noir isolates by restriction fragment length polymorphism of a stolbur-specific putative membrane protein gene
Phytopathology 99 (6), 711-715
Abstract: Bois noir phytoplasma (BNp), widespread in wine-producing areas of Europe and endemic in France and Italy, is classified in the 16SrXII-A subgroup, whose members are referred to as Stolbur phytoplasmas. The 16S rDNA gene of Stolbur phytoplasma shows low variability, and few non-ribosomal genes are available as markers to assess variation among isolates. We used the Stolbur-specific stol-1H10 gene, encoding a putative membrane-exposed protein, to investigate genetic diversity of French and Italian BNp isolates from plants and insects. Amplification of stol-1H10 from infected grapevines, weeds, and Hyalesthes obsoletus produced fragments of three sizes, and restriction fragment length polymorphism analysis divided these amplicons further into 12 profiles (V1 to V12). French BNp isolates were more variable than Italian ones, and different profiles were present in infected grapevines from France and Italy. Isolate V3, most abundant among Italian affected grapes but present among French ones, was found in one Urtica dioica sample and in all H. obsoletus collected on this species. Four Italian-specific profiles were represented among infected Convolvulus arvensis, the most frequent of which (V12) was also detected in H. obsoletus collected on this species. Most of the variability in the stol-1H10 sequence was associated with type II on the tuf gene.
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Full text of article
Database assignments for author(s): C. Marzachi, Davide Pacifico, Alberto Alma, Xavier Foissac

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
environment - cropping system/rotation


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Phytoplasma solani Grapevine (Vitis) France
Phytoplasma solani Grapevine (Vitis) Italy