Phytopathology (2008) 98, 1019-1028

From Pestinfo-Wiki
Jump to: navigation, search

M. Collado-Romero, J. Mercado-Blanco, C. Olivares-García and R.M. Jiménez-Díaz (2008)
Phylogenetic analysis of Verticillium dahliae vegetative compatibility groups
Phytopathology 98 (9), 1019-1028
Abstract: The evolutionary relationships among Verticillium dahliae vegetative compatibility (VCG) subgroups VCG1A, VCG1B, VCG2A, VCG2B, VCG4A, VCG4B, and VCG6 were investigated by parsimony analysis of amplified fragment length polymorphism (AFLP) fingerprints and sequences of six DNA regions (actin, ß-tubulin, calmodulin, and histone 3 genes, the ITS 1 and 2 regions of the rDNA, and a V. dahliae-specific sequence), using 101 isolates of diverse host and geographic origin. Polymorphisms in gene sequences among isolates of different VCGs were very low and individual gene genealogies provided very little resolution at the VCG level. The combined analysis of all DNA regions differentiated all VCG subgroups except for isolates in VCG1A and VCG1B. VCG clonal lineages in V. dahliae and evolutionary relationships among them were resolved independently by analyses of AFLP fingerprints, multiple gene genealogies, and the combined data set of AFLP fingerprinting and multiple gene genealogies. Two main lineages (I and II) were identified with lineage II comprising two closely related subgroups of VCGs. Lineage I included VCG1A, VCG1B, and VCG2B334; and lineage II included, VCG2A and VCG4B (subclade 1); and VCG2B824, VCG4A, and VCG6 (subclade 2). VCG subgroups were monophyletic except for VCG2B that appeared polyphyletic. Limiting the parsimony analysis either to AFLP fingerprints or DNA sequences would have obscured intra-VCG differentiation. Therefore, the dual approach represented by the independent and combined analyses of AFLP fingerprints and DNA sequences was a highly valuable method for the identification of phylogenetic relationships at the intraspecific level in V. dahliae.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Rafael M. Jimenez-Diaz, Jesús Mercado-Blanco, Melania Collado-Romero

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Verticillium dahliae Almond (Prunus dulcis) Spain (continental)
Verticillium dahliae Cabbage (Brassica oleracea) Germany
Verticillium dahliae Eggplant (Solanum melongena) Israel
Verticillium dahliae Green pepper/chilli (Capsicum) U.S.A. (SW)
Verticillium dahliae Potato (Solanum tuberosum) Spain (continental)
Verticillium dahliae Potato (Solanum tuberosum) Israel
Verticillium dahliae Strawberry (Fragaria) United Kingdom
Verticillium dahliae Tomato (Solanum lycopersicum) Italy
Verticillium dahliae Tomato (Solanum lycopersicum) Israel
Verticillium dahliae Artichoke (Cynara) Spain (continental)
Verticillium dahliae Olive (Olea europaea) Cyprus
Verticillium dahliae Olive (Olea europaea) Greece
Verticillium dahliae Olive (Olea europaea) Italy
Verticillium dahliae Olive (Olea europaea) Spain (continental)
Verticillium dahliae Cotton (Gossypium) Greece
Verticillium dahliae Cotton (Gossypium) Spain (continental)
Verticillium dahliae Cotton (Gossypium) Turkey
Verticillium dahliae Cotton (Gossypium) U.S.A. (SW)
Verticillium dahliae Cotton (Gossypium) Israel
Verticillium dahliae Melon (Cucumis melo) Spain (continental)
Verticillium dahliae Chickpea (Cicer arietinum) Italy
Verticillium dahliae Watermelon/colocynth (Citrullus) Spain (continental)
Verticillium dahliae Squash/pumpkin (Cucurbita) Spain (continental)