Phytopathology (1996) 86, p. S45 (Cui et al.)

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Y. Cui, A.A. Bell and C. Magill (1996)
Differential induction of cotton defense pathways by Verticillium; cloning defense response genes
Phytopathology 86 (11 suppl.), S45-S45
Abstract: By comparing sequences from distantly related species, we identified short, highly conserved sequences that flank less conserved regions of several defense related genes. Primers designed in this fashion were used with PCR to amplify segments of cotton DNA that were expected to code for PAL, o-methyltransferase, cinnamyl-alcohol dehydrogenase, chitinase, glucanase, HMGCoA reductase, chalcone synthase, glutamine synthetase and dihydroflavonol reductase. The amplified fragments were cloned into pUC18 in E. colt. Nucleotide sequences were confirmed by showing >70% sequence homology within the coding region to the equivalent genes from other species. The clones then were used to probe Northern blots that contain mRNA following introduction of Verticillium dahliae spores into the vascular system. The primary results showed that flavonoid, terpenoid and lignin pathways may be involved in cotton defense. The timing and amount of defense gene mRNA accumulation after the Verticillium infection varied among different cotton cultivars.
Database assignments for author(s): Clint Magill

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Verticillium dahliae Cotton (Gossypium)