Phytopathology (1996) 86, p. S35 (Kumari et al.)

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S.R. Kumari, C.W. Magill and R.A. Frederiksen (1996)
Identification of molecular markers for grain mold resistance and mapping of fungal inhibiting protein genes in sorghum (Sorghum bicolor (L.) Moench)
Phytopathology 86 (11 suppl.), S35-S35
Abstract: Little research has been conducted at the molecular level on sorghum grain mold resistance. Availability of tightly linked markers would make the screening procedures easier and more accurate. Two sorghum variety resistant (Sureno) and susceptible (SC-120) to grain molds were selected. The obtained results revealed that the crushing strength of grains of the variety Sureno was higher when compared to the susceptible variety SC-120. The incidence of F. moniliforme was found to be 68% in the susceptible variety and 15% in the resistant variety. The PCR-RAPD technique was used to obtain markers linked to grain mold resistance. Four primers (OPA12, OPNOS, OPR10, and OPR11) showed fragments segregating with resistance. The size of fragments amplified by OPN05 were 1.6, 1.0 and 0.3 kb. A single fragment was linked to susceptibility, the size of which was found to be 0.5 kb. The RNA studies revealed that the level of OPN05-1R increases after infection with F. moniliforme. Different andfungal genes were placed on the sorghum RFLP map.
Database assignments for author(s): Clint Magill

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Fusarium verticillioides Sorghum (crop)