Phytopathology (1996) 86, p. S29 (Strydom et al.)
R.C. Strydom, B.D. Wingfield, M.J. Wingfield and T.C. Harrington (1996)
Identification of species of Ceratocystis sensu stricto using RFLP and DNA sequence data
Phytopathology 86 (11 suppl.), S29-S29
Abstract: Most species of Ceratocystis sensu stricto are virulent pathogens of a wide variety of plants including forest and fruit trees, sweet potatoes, pineapples and sugar cane. These organisms are difficult to identify. In this study we have developed a quick and reliable method to distinguish between 10 different species of Ceratocystis. A 1600 base pair fragment within the ribosomal DNA operon was amplified using the polymerase chain reaction (PCR). The amplification was done directly from living fungal tissue without extracting DNA. The amplified fragment included part of the small and large sub-unit rRNA genes, the 5.8S rRNA gene and the internal transcribed spacers (ITS) 1 and 2. We sequenced both strands of the 1600 base pair PCR product of one isolate of each of the species. Phylogenetic relatedness was determined using Phylogenetic Analysis Using Parsimony (PAUP). We were unable to distinguish most of the Ceratocystis species based on the unique restriction fragments produced by Alul. RFLP and sequence data were thus used in the development of a rapid and accurate diagnostic tool to identify species of Ceratocystis.
Database assignments for author(s): Brenda D. Wingfield, Michael J. Wingfield, Thomas C. Harrington
Research topic(s) for pests/diseases/weeds:
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