Phytopathology (1996) 86, p. S19 (Peever et al.)

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T.L. Peever and M.G. Milgroom (1996)
Genetic diversity of double-stranded RNAs infecting North American populations of the chestnut blight fungus, Cryphonectria parasitica
Phytopathology 86 (11 suppl.), S19-S19
Abstract: Six hundred isolates of the chestnut blight fungus, Cryphonectria parasitica, were randomly sampled from 9 geographically separated subpopulations in North America and screened for the presence of double-stranded RNA (dsRNA) using a monoclonal antibody specific for dsRNAs. Approximately 28% of all isolates were infected with dsRNA. The genetic relatedness of dsRNAs within and among 7 subpopulations was analyzed using Northern hybridizations with dsRNA probes randomly selected from the different subpopulations. dsRNAs from Michigan, New York, New Jersey, Maryland and West Virginia formed a single group that cross-hybridized to SR2 dsRNA found previously in West Virginia. dsRNAs from 2 populations in Michigan formed another group that cross-hybridized to Cryphonectria hypovirus 3 (CHV3). dsRNAs in Frankfort, Michigan hybridized to both SR2 and CHV3 and was subsequently shown to be caused by mixed infections.
Database assignments for author(s): Michael G. Milgroom

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
surveys/sampling/distribution
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Cryphonectria parasitica
Cryphonectria parasitica hypoviruses (antagonist) Cryphonectria parasitica U.S.A. (NE)