Phytoparasitica (2005) 33, p. 285 (Antignus et al.)

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Y. Antignus, L. Feigelson, Mali Pearlsman, O. Lachman, H. Yunis and A. Gipps (2005)
Adaptation of a disinfection system, based on chlorination, to eliminate contamination of greenhouse drainwater with soilborne viruses
Phytoparasitica 33 (3), 285-285
Abstracts of presentations at the 26th Congress of the Israeli Phytopathological Society (February 14-15, 2005, Bet Dagan, Israel), lecture
Abstract: The acute shortage in water supply for agriculture, the prohibition on the use of methyl bromide and the awareness of the need to prevent environmental pollution, encouraged the expansion of soilless greenhouse cultures and the recycling of the drainwater that is generated in these systems. Greenhouse cucumbers grown in Israel are affected by two soilborne viral pathogens. Cucumber fruit mottle mosaic virus (CFMMV) and Cucumber leaf spot virus (CLSV), which are known for their high survival in soil and water and therefore the re-circulation of drainwater may play a role in their spread in soilless media. The installation of a disinfection unit into the recycling system may help to prevent this process. This work was aimed at studying some parameters affecting the epidemiology of soilborne viruses in soilless cultures and at calibrating a chlorination system that is directed to prevent the accumulation of viral inoculum in the drainwater and the growth medium. An inoculum threshold of 10 µg ml-1 is required to produce CFMMV infection through the root system of cucumbers, as was determined in plastic pots using a purified preparation of CFMMV. The adsorption of purified CFMMV to soil, perlite and tuff was measured in columns, showing that perlite has the highest specific adsorption capacity while lower values were calculated for soil and tuff, respectively. The specific adsorption capacity of CFMMV in the three tested media was much higher than the inoculum threshold found in our lab experiments. Purified CFMMV inoculum survived in the tested media under greenhouse conditions for at least 4 months. Analysis of drainwater collected from a commercial greenhouse indicated the presence of infectious CFMMV and CLSV inocula, despite the application of chlorine into the circulated water. Lab tests showed that efficient disinfection of drainwater contaminated with CFMMV or CLSV at a concentration of 1 µg ml-1 is obtained by chlorination with 4 ppm hypoclorite for 4 h. The efficiency of chlorination in drainwater decreases dramatically in the presence of fertilizer ions.


Research topic(s) for pests/diseases/weeds:
control - general


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Cucumber fruit mottle mosaic virus Cucumber (Cucumis sativus) Israel
Cucumber leaf spot virus Cucumber (Cucumis sativus) Israel