Phytoparasitica (2001) 29, 103-113

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Marcelo A.B. Morandi, Luiz A. Maffia and John C. Sutton (2001)
Development of Clonostachys rosea and interactions with Botrytis cinerea in rose leaves and residues
Phytoparasitica 29 (2), 103-113
Abstract: The effect of microclimate variables on development of Clonostachys rosea and biocontrol of Botrytis cinerea was investigated on rose leaves and crop residues. C. rosea established and sporulated abundantly on inoculated leaflets incubated for 7–35 days at 10° , 20° and 30° C and then placed on paraquat–chloramphenical agar (PCA) for 15 days at 20° C. On leaflets kept at 10° C, the sporulation after incubation on PCA increased from 60% to 93% on samples taken 7 to 21 days after inoculation, but decreased to 45% on material sampled after 35 days. A similar pattern was observed on leaves incubated at either 20° or 30° C. The sporulation of C. rosea on leaf disks on PCA was not affected when the onset of high humidity occurred 0, 4, 8, 12 or 16 h after inoculation. However, sporulation was reduced to 54–58% on leaflets kept for 20–24 h under dry conditions after inoculation and before being placed on PCA. The fungus sporulated on 68–74% of the surface of leaf disks kept for up to 24 h at high humidity after inoculation, but decreased to 40–51% if the high humidity period before transferral to PCA was prolonged to 36–48 h. The growth of C. rosea on leaflets was reduced at low inoculum concentrations (103 and 104 conidia/ml) because of competition with indigenous microorganisms, but at higher concentrations (105 and 106 conidia/ml) the indigenous fungi were inhibited. Regardless of the time of application of C. rosea in relation to B. cinerea, the pathogen's sporulation was reduced by more than 99%. The antagonist was able to parasitize hyphae and conidiophores of B. cinerea in the leaf residues. As C. rosea exhibited flexibility in association with rose leaves under a wide range of microclimatic conditions, and in reducing B. cinerea sporulation on rose leaves and residues, it can be expected to suppress the pathogen effectively in rose production systems.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Marcelo A.B. Morandi, Luiz A. Maffia

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
environment/habitat manipulation


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Botrytis cinerea
Clonostachys rosea (antagonist) Botrytis cinerea