Phytoparasitica (2000) 28, 141-148

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Christina Varveri (2000)
Potato Y potyvirus detection by immunological and molecular techniques in plants and aphids
Phytoparasitica 28 (2), 141-148
Abstract: An antiserum against potato Y potyvirus (PVY) was produced and the double antibody sandwich (DAS)-ELISA technique with a detection sensitivity of 10 ng/ml purified virus, was applied. One-step immunocapture (IC) – reverse transcription (RT) – polymerase chain reaction (PCR) was assessed using published primers; a gain in sensitivity of 1000-fold over ELISA was achieved. Furthermore, the use of PCR-ELISA to detect the produced amplicons after their direct adsorption on a microplate offered a supplementary 100-fold gain in sensitivity. Thus, 100 fg/ml purified virus in healthy tobacco extract became readily detectable, as did an infected potato sample at a dilution end point of 10-7, equivalent to 1 ng tissue. This highly sensitive procedure coupled with the print-capture (PC) modification to avoid specific sample preparation, offered a most handy and useful tool for effective PVY detection in single aphids (Myzus persicae).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Christina Varveri

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Potato virus Y Potato (Solanum tuberosum)