Phytoparasitica (1997) 25, p. 75 (Qiu et al.)

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W.P. Qiu and J.W. Moyer (1997)
Directed adaptation of tomato spotted wilt tospovirus (TSWV) by reassortment on resistant plants
Phytoparasitica 25 (1), 75-75
The Xth International Congress of Virology, August 11-16, 1996, Jerusalem, Israel, poster
Abstract: The biological and genetic diversity of TSWV may be shaped by a combination of reassortment of tripartite genomic segments (L, M, S) and selective host genetics in addition to mutation and recombination. In this study we demonstrated that transgenic resistance is a selective force which drives adaptive reassortment of genomic segments, resulting in the generation of a new strain capable of overcoming resistance. In order to determine whether a viral population could be directed by resistant plants to form a resistance-breaking strain through reassortment, two parental isolates, TSWV-D and TSWV-10, were co-inoculated on normal Nicotiana tabacum cv. Burley 21 (susceptible) and transgenic Burley 21 (resistant). Neither of the parental strains was capable of overcoming the resistance. The genomic segments from each strain were monitored following each sequential transfer. The viral population remained a mixture on the susceptible tobacco after seven transfers. However, the mixed population was driven to form a pure reassortant, L10M10SD, on the resistant line. Our experiments revealed that genomic reassortment is a mechanism utilized by TSWV to overcome host resistance.
Database assignments for author(s): Wenping Qiu

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Tomato spotted wilt virus