Mycologia (2004) 96, 1352-1354

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Paal Krokene, Irene Barnes, Brenda D. Wingfield and Michael J. Wingfield (2004)
A PCR-RFLP based diagnostic technique to rapidly identify Seiridium species causing cypress canker
Mycologia 96 (6), 1352-1354
Abstract: Seiridium cardinale, S. cupressi and S. unicorne represent three distinct species of fungi that cause cankers on Cupressus species and the disease collectively known as cypress canker. These fungi cannot be distinguished reliably from each other using morphological characters or ribosomal DNA sequence data. Here we describe a RFLP assay based on digesting ß-tubulin amplicons with a single endonuclease, HaeIII, which easily can be used to distinguish among these three species. This RFLP assay provides an inexpensive and simple means of identifying Seiridium species, which include some of the most serious threats to trees in Cupressaceae.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Paal Krokene, Brenda D. Wingfield, Michael J. Wingfield, Irene Barnes

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Seiridium cardinale Cypress (Cupressus)
Seiridium unicorne Cypress (Cupressus)