Molecular Plant-Microbe Interactions (2019) 32, 142-156

From Pestinfo-Wiki
Jump to: navigation, search
People icon1.svgSelected publication
of interest to a wider audience. We would welcome
contributions to the Discussion section (above tab) of this article.
Remember to log in or register (top right corner) before editing pages.
Alberto Carbonell, Carmelo López and José-Antonio Daròs (2019)
Fast-forward identification of highly effective artificial small RNAs against different Tomato spotted wilt virus isolates
Molecular Plant-Microbe Interactions 32 (2), 142-156
Abstract: Artificial small RNAs (sRNAs), including artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are used to silence viral RNAs and confer antiviral resistance in plants. Here, the combined use of recent high-throughput methods for generating artificial sRNA constructs and the Tomato spotted wilt virus (TSWV)–Nicotiana benthamiana pathosystem allowed for the simple and rapid identification of amiRNAs with high anti-TSWV activity. A comparative analysis between the most effective amiRNA construct and a syn-tasiRNA construct including the four most effective amiRNA sequences showed that both were highly effective against two different TSWV isolates. These results highlight the usefulness of this high-throughput methodology for the fast-forward identification of artificial sRNAs with high antiviral activity prior to time-consuming generation of stably transformed plants.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Database assignments for author(s): José-Antonio Daròs, Carmelo Lopez

Research topic(s) for pests/diseases/weeds:
control - general

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.
Tomato spotted wilt virus