Molecular Plant-Microbe Interactions (2005) 18, 60-66

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Chun-Mei Li, Minna Haapalainen, Justin Lee, Thorsten Nürnberger, Martin Romantschuk and Suvi Taira (2005)
Harpin of Pseudomonas syringae pv. phaseolicola harbors a protein binding site
Molecular Plant-Microbe Interactions 18 (1), 60-66
Abstract: Harpin HrpZ of plant-pathogenic bacterium Pseudomonas syringae elicits a hypersensitive response (HR) in some nonhost plants, but its function in the pathogenesis process is still obscure. HrpZ-interacting proteins were identified by screening a phage-display library of random peptides. HrpZ of the bean pathogen P. syringae pv. phaseolicola (HrpZ(Pph)) shows affinity to peptides with a consensus amino acid motif W(L)ARWLL(G/L). To localize the peptide-binding site, the hrpZ(Pph) gene was mutagenized with randomly placed 15-bp insertions, and the mutant proteins were screened for the peptide-binding ability. Mutations that inhibited peptide-binding localized to the central region of hrpZ(Pph), which is separate from the previously determined HR-inducing region. Antiserum raised against one of the hrpZ(Pph)-binding peptides recognized small proteins in bean, tomato, parsley, and Arabidopsis thaliana but none in tobacco. On native protein blots, hrpZ(Pph) bound to a bean protein with similar pI as the protein recognized by the peptide antiserum. The result suggests a protein-protein interaction between the harpin and a host plant protein, possibly involved in the bacterial pathogenesis.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Minna Haapalainen

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
resistance/tolerance/defence of host

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Pseudomonas syringae pv. phaseolicola
Pseudomonas syringae