Letters in Applied Microbiology (2001) 33, 397-401
P.-H. Wang, H.S. Lo and Y. Yeh (2001)
Identification of F. o. cucumerinum and F. o. luffae by RAPD-generated DNA probes
Letters in Applied Microbiology 33 (5), 397-401
Abstract: Aims: To create a fast, sensitive and specific method for identifying Fusarium oxysporum f. sp. cucumerinum and F. o. luffae.
Methods and Results: Specific DNA bands were selected as probes from RAPD profiles of 13 formae speciales of F. oxysporum. The forma specialis-specific probe OPC18300c and OPC18520f could be used to identify F. o. cucumerinum and F. o. luffae by RAPD-PCR followed dot blot hybridization, respectively
Conclusions: A specific method for identifying F. o. cucumerinum and F. o. luffae was achieved.
Significance and Impact of the Study: F. oxysporum formae speciales identification with a DNA probe can be relatively rapid and provides a method to identify the pathogen without host inoculation tests.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Research topic(s) for pests/diseases/weeds:
Pest and/or beneficial records:
|Fusarium oxysporum f.sp. cucumerinum||Taiwan|
|Fusarium oxysporum f.sp. luffae||Taiwan|