Letters in Applied Microbiology (1998) 27, 152-157

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N. Rajendran, A. Rompf, M.A. Marahiel and D. Jahn (1998)
Isolation and characterization of a transposon mutant of Pseudomonas fluorescens AU63 deficient in antifungal activity against Pythium ultimum
Letters in Applied Microbiology 27 (3), 152-157
Abstract: Tn5 transposon mutagenesis via electroporation of Pseudomonas fluorescens AU63 was used to generate mutants deficient in antifungal activity against the phytopathogenic fungi Pythium ultimum and Thielaviopsis basicola. Mutant C-45 was obtained by an initial screen for the loss of antibacterial activity against Bacillus subtilis and a subsequent screen of mutants obtained for the loss of antifungal activity against pathogenic fungi. A single chromosomal insertion of Tn5 in the chromosome of Ps. fluorescens C-45 was confirmed by Southern blot hybridization. A metabolite responsible for the observed antibacterial and antifungal activities was identified using thin layer chromatography. The antimicrobial activities of the partially purified substance present in the parental strain and missing in the C-45 mutant were not affected by protease, high temperature, acid or alkali treatment. These results provide the basis for a structural analysis of this new antimicrobial substance and the genetic elucidation of its biosynthesis.
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Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Berkeleyomyces basicola
Globisporangium ultimum
Pseudomonas fluorescens (antagonist) Berkeleyomyces basicola
Pseudomonas fluorescens (antagonist) Globisporangium ultimum