Letters in Applied Microbiology (1998) 26, 456-462

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C. Grimm and R. Geisen (1998)
A PCR-ELISA for the detection of potential fumonisin producing Fusarium species
Letters in Applied Microbiology 26 (6), 456-462
Abstract: A PCR-ELISA for the detection of potential fumonisin producing Fusarium species has been developed, using the ribosomal ITS1 sequence as target. For this purpose, the sequences of the ITS1 regions of different fumonisin producing Fusarium species have been determined and compared to the sequences of fumonisin non-producing species. In general, the ITS1 sequences were highly homologous. However, some minor sequence polymorphisms were detected, which differentiates potential fumonisin producing Fusarium species from non-producing species. By using these sequence differences, a PCR-ELISA for potential fumonisin producing Fusarium species was developed. All other ubiquitously occurring food-borne fungi tested showed negative results with this test.
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Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Fusarium (genus)
Fusarium culmorum