Letters in Applied Microbiology (1998) 26, 387-390

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J.H. Chang, J.Y. Roh, Y.H. Je, H.W. Park, B.R. Jin, S.D. Woo and S.K. Kang (1998)
Isolation of a strain of Bacillus thuringiensis ssp. kurstaki HD-1 encoding delta-endotoxin Cry1E
Letters in Applied Microbiology 26 (5), 387-390
Abstract: A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference strains, ssp. kurstaki and ssp. kenyae. To verifiy the gene type of B. thuringiensis STB-1, PCR analysis was performed with Spodoptera-specific cry gene primers. The result showed that B. thuringiensis STB-1, unlike its reference strains, had cry1Aa, cry1Ab, cry1Ac and cry1E, suggesting that B. thuringiensis STB-1 was a unique strain with respect to gene type. In addition, B. thuringiensis STB-1 showed a high level of toxicity against both S. exigua and Bombyx mori, whereas B. thuringiensis ssp. kurstaki HD-1 or ssp. kenyae showed a high level of toxicity against only Bombyx mori or S. exigua, respectively.
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Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Spodoptera exigua Korea-South
Bacillus thuringiensis var. kurstaki (entomopathogen) Spodoptera exigua Korea-South
Bacillus thuringiensis Cry1A-toxin (entomopathogen) Spodoptera exigua Korea-South
Bacillus thuringiensis Cry1E-toxin (biocontrol agent) Spodoptera exigua Korea-South