Journal of the American Society for Horticultural Science (2009) 134, 529-534

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Karen R. Harris, Kai-Shu Ling, W. Patrick Wechter and Amnon Levi (2009)
Identification and utility of markers linked to the Zucchini yellow mosaic virus resistance gene in watermelon
Journal of the American Society for Horticultural Science 134 (5), 529-534
Abstract: Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses affecting watermelon [Citrullus lanatus (Thunb.) Matsun and Nakai var. lanatus] in the United States. The ZYMV-Florida strain (ZYMV-FL) is considered a major limitation to commercial watermelon production in the United States. Inheritance of resistance to ZYMV-FL is conferred by a recessive gene. This report describes the identification of single-reaction, polymerase chain reaction-based markers linked to the ZYMV-FL resistance gene in watermelon. In this study, we identified a marker ZYMV-resistant polymorphism (ZYRP) linked to the ZYMV-FL resistance gene locus (genetic distance of 8 cM) in an F2 population, and in a backcross one to the resistant parent population (BC1R) (genetic distance of 13 cM). The identification of a single nucleotide polymorphism within the ZYRP marker for the parental genotypes allowed the development of a sequence-characterized amplification region marker linked to the ZYMV-FL resistance gene. Experiments using a BC2F2 population derived from the U.S. Plant Introduction 595203 (C. lanatus var. lanatus) and the recurrent parent 'Charleston Gray' indicated that the ZYRP marker can be used in marker-assisted selection to identify genotypes containing the gene conferring ZYMV-FL resistance in watermelon.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Kai-Shu Ling

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Zucchini yellow mosaic virus Watermelon/colocynth (Citrullus)