Journal of Plant Protection Research (2006) 46, 325-334

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Elzbieta U. Kozik, Joanna Pulawska and Piotr Sobiczewski (2006)
Genetic similarity of Pseudomonas syringae pv. tomato strains showing various virulence
Journal of Plant Protection Research 46 (4), 325-334
Abstract: Bacterial speck of tomato caused by Pseudomonas syringae pv. tomato appeared to be recently the most important disease on tomato in Poland. The genetic relationships among four Polish strains of race 0 P. syringae pv. tomato of different origin, isolated from tomato plants, were examined by RAPD and PCR-RFLP techniques. Amplification of bacterial DNA using 33 primers with RAPD technique showed, that similarity of strains expressed by the Nei-Li coefficient was very high (above 0.8). Next, the restriction analysis of amplified region ITS with the use of 5 endonucleases revealed, that profiles obtained from electrophoretic separation of DNA fragments were also very similar. On the basis of those analyses it was concluded that all strains tested constituted a closely related group. However, they showed various level of virulence as was demonstrated on the inoculated leaves of tomato plants growing in the greenhouse.
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Database assignments for author(s): Elzbieta U. Kozik, Joanna Pulawska

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Pseudomonas syringae pv. tomato Tomato (Solanum lycopersicum) Poland
Pseudomonas syringae