Journal of Plant Protection Research (1996) 37, 52-58

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Mieczyslaw Cajza, Krzysztof Wypijewski and Henryk Pospieszny (1996)
The use of reverse transcription and polymerase chain reaction (RT-PCR) for the detection of Hop latent viroid (HLVd)
Journal of Plant Protection Research 37 (1-2), 52-58
Abstract: The simple method of nucleic acids extraction, based on guanidine thiocyanate extraction buffer, was sufficient for obtaining a good templates for RT-PCR. The RT-PCR reactions were performed from the start to the end (both the reverse transcription and the HLVd-cDNA amplification) in the same reaction mixture and in the very small volume of reaction (10 µl). Both pairs of primers designed by authors were good for reverse transcription and later for amplification of the HLVd-cDNA. The presence of gelatin as a stabilizer of DNA polymerase was indispensable for successful performance of RT-PCR.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Henryk Pospieszny

Research topic(s) for pests/diseases/weeds:
identification/taxonomy


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Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Hop latent viroid