Journal of Plant Pathology (2007) 89, p. S56 (Puopolo et al.)

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G. Puopolo, A. Raio and A. Zoina (2007)
First insight on the possible correlation between bacteriolytic activity and biofilm formation in a Lysobacter sp. strain
Journal of Plant Pathology 89 (3, Suppl.), S56-S56
Poster Presentation at S.I.Pa.V XIV National Meeting - Perugia, Italy 19-21 Sept. 2007
Abstract: Most members of the genus Lysobacter possess high bacteriolityc activity against both Gram-negative and Gram-positive bacteria. Lysobacter sp. strain PG4 was evaluated on different growth media for its antagonistic activity towards 16 strains belonging to different bacterial species. The highest bacteriolytic activity was observed against bacterial species belonging to the Gram-positive group such as Clavibacter michiganensis subsp. michiganensis, Rhodococcus fascians and Listeria monocytogenes while no activity was scored against Gram-negative bacteria. The bacteriolytic activity was affected by growth media. King's B Medium (KBM) was the only one that affected negatively the antibiotic capacity of strain PG4. Interestingly, the addition of FeCl3 to this medium restored PG4 bacteriolytic activity indicating that Fe3+ ions are involved in this activity. Some of the growth media used in bacteriolytic tests were also used in adhesion assays performed in 96 wells polystyrene plates, a common test that allows to evaluate the production of biofilm by bacteria. The ability of Lysobacter sp. strain PG4 to form biofilm was correlated to the entry in the stationary phase, except when the bacterium was cultivated in KBM. By contrast, strain PG4 was unable to form biofilm at all. These results represent a first insight on the correlation between biofilm formation and bacteriolytic activity in a member of the genus Lysobacter.
Database assignments for author(s): Gerardo Puopolo, Aida Raio

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
rearing/culturing/mass production


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Clavibacter michiganensis
Rhodococcus fascians
Lysobacter (genus - antagonists) Clavibacter michiganensis
Lysobacter (genus - antagonists) Rhodococcus fascians