Journal of Plant Pathology (2007) 89, p. S50 (Nigro et al.)

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F. Nigro, I. Pentimone, H. Barham and A. Ligorio (2007)
Partial characterization of the fow1 gene in Verticillium dahliae isolates from olive trees
Journal of Plant Pathology 89 (3, Suppl.), S50-S50
Poster Presentation at S.I.Pa.V XIV National Meeting - Perugia, Italy 19-21 Sept. 2007
Abstract: Verticillium dahliae Kleb., the causal agent of a vascular wilt disease, is an economically important plant pathogen with worldwide distribution, affecting severely young olive trees. To date, the molecular mechanisms of pathogenicity and symptom induction by V. dahliae in olive trees remain largely unexplored, although some pathogenicity genes of the fungus have been recently identified and characterized. Improving the knowledge on V. dahliae pathogenicity could be useful for epidemiological studies and for developing effective control strategies. Using primers (gi-Fow1F-giFowR), designed on a conserved hypothetical protein of Gibberella zeae (XM381597), a 550 bp DNA fragment (VerFow1) was amplified from DNA extracted from several V. dahliae isolates, cloned and sequenced. Basic local alignment search tool (BLAST) of the fragment VerFow1 showed a considerable homology with a yeast and fungal hypothetical protein related to a mitochondrial carrier protein (MCPs). This protein is known for its role in communication between mitochondrial matrix and cytosol, being essential in eukaryotic metabolism. In particular, sequence analysis revealed a 96% identity with the corresponding region of Fusarium oxysporum FOW1 gene (AB078975), encoding a mitochondrial protein that was identified and characterized as a virulence determinant, specifically required for the colonization of melon plants. Trials to determine the role of the fragment VerFow1 in the pathogenesis of V. dahliae on olive trees are in progress. To the best of our knowledge this is the first report on the characterization of a fungal hypothetical protein related to mitochondrial carriers in V. dahliae.
Database assignments for author(s): Franco Nigro

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Verticillium dahliae Olive (Olea europaea)