Journal of Phytopathology - Phytopathologische Zeitschrift (2003) 151, 535-539

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T. Kon, L.M. Dolores, N.B. Bajet, S. Hase, H. Takahashi and M. Ikegami (2003)
Molecular characterization of a strain of Squash leaf curl China virus from the Philippines
Journal of Phytopathology - Phytopathologische Zeitschrift 151 (10), 535-539
Abstract: The complete nucleotide sequence of infectious cloned DNA components (A and B) of the causal agent of squash leaf curl disease in the Philippines was determined. DNA-A and DNA-B comprise 2739 and 2705 nucleotides, respectively; the common region is 174 bases in length. Five ORFs were found in DNA-A and two in DNA-B. Partial dimeric clones containing DNA-A and DNA-B, constructed in a binary vector and transformed into Agrobacterium tumefaciens, induced systemic infection in agro-inoculated pumpkin plants (Cucurbita moschata). The total DNA-A sequence was most closely related to that of Squash leaf curl China virus (SLCCNV) (88% identity), although the existence of B component of SLCCNV has not been reported. The deduced coat protein was like that of SLCCNV (98% amino acid sequence identity) and the Philippines virus has low sequence identity to Squash leaf curl virus (SLCV) and Squash mild leaf curl virus (SMLCV) (63 and 64% total nucleotide sequence identities, respectively). From these results, we propose that the Philippines virus be designated Squash leaf curl China virus-[Philippines] (SLCCNV-[PH]).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Masato Ikegami, Hideki Takahashi

Research topic(s) for pests/diseases/weeds:
molecular biology - genes


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Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Squash leaf curl China virus Philippines