Journal of Phytopathology (2015) 163, 657-663

María I. Dinolfo, Eliana Castañares and Sebastián A. Stenglein (2015)
SRAP as an informative molecular marker to study the Fusarium poae genetic variability
Journal of Phytopathology 163 (7-8), 657-663
Abstract: Fusarium poae is one of the Fusarium species isolated from grains associated with Fusarium head blight (FHB), whose occurrence has increased in the last years. In this study, a total of 105 F. poae isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated using sequence-related amplified polymorphism (SRAP) to analyse the capacity of this molecular marker to evaluate the F. poae genetic variability. The molecular analysis showed high intraspecific variability within F. poae isolates, and a partial relationship was revealed between variability and the host/geographic origin. Analysis of molecular variance (amova) indicated a high genetic variability in the F. poae collection, with most of the genetic variability resulting from differences within, rather than between American and European populations. The analysis of sequenced SRAP fragments targets into hypothetical proteins from different Fusarium species showing that the SRAP technique not only allows studying F. poae genetic variability, but also targets coding regions into the F. poae genome. To our knowledge, this is the first report on genetic variability of F. poae using SRAP technique and also demonstrates the efficacy of this molecular marker to amplify open reading frames in fungus.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium poae		Belgium
	Fusarium poae		Finland
	Fusarium poae		France
	Fusarium poae		Germany
	Fusarium poae		Hungary
	Fusarium poae		Italy
	Fusarium poae		Luxembourg
	Fusarium poae		Poland
	Fusarium poae		Switzerland
	Fusarium poae		United Kingdom
	Fusarium poae		Argentina
	Fusarium poae		Uruguay