Journal of Phytopathology (2010) 158, 496-502

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Krzysztof Krawczyk, Henryk Pospieszny and Joanna Kamasa (2010)
Identification of new members of Candidatus Phytoplasma asteris affecting tomato plants in Poland
Journal of Phytopathology 158 (7-8), 496-502
Abstract: Two symptomatic tomato plants exhibiting dwarfing, twisting of shoots and leaves, virescence and phyllody of flowers were collected, respectively, from a greenhouse (Soly07fi) or the field (Soly06gh) in the western region of Poland. Direct and nested polymerase chain reactions (PCR) were performed using universal phytoplasma primers P1/P7 and R16F2n/R16R2. Restriction fragment length polymorphism (RFLP) analysis of the PCR products showed that the RFLP profiles of both tested phytoplasma isolates are the same and that they belong to the phytoplasma 16S rRNA I-C subgroup. The homology between the two strains was 99%. Phylogenetic analysis of the 16S rRNA gene sequences of the phytoplasma isolates and other phytoplasma sequences available in the GenBank database indicated that the Polish phytoplasma isolates are most closely related to the phytoplasma 16S rRNA I-C subgroup.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Krzysztof Krawczyk, Henryk Pospieszny

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Phytoplasma asteris Tomato (Solanum lycopersicum) Poland