Journal of Phytopathology (2007) 155, 65-69

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K.-S. Ling, H.-Y. Zhu, N. Petrovic and D. Gonsalves (2007)
Serological detection of Grapevine leafroll virus 2 using an antiserum developed against the recombinant coat protein
Journal of Phytopathology 155 (2), 65-69
Abstract: Grapevine leafroll associated virus 2 (GLRaV 2) is one of the important components in the leafroll disease complex. The coat protein gene of GLRaV 2 was cloned into a protein expression vector pMAL-c2x and the recombinant protein, consisting of the maltose binding protein (MBP) and GLRaV 2 coat protein (CP), was expressed in Escherichia coli. The recombinant MBP-CP was used to raise a high quality antiserum. When used in Western blot analysis, the anti-MBP-CP antiserum produced specific reaction to the recombinant protein as well as to the viral coat protein of GLRaV 2. In Immunosorbent electron microscopy study, the anti-MBP-CP antibodies strongly decorated the GLRaV 2 virions. Using the newly developed antiserum, an indirect plate-trapped antigen enzyme-linked immunosorbent assay method was developed and successfully implemented for virus detection. A field survey was conducted to evaluate the virus infection status by GLRaV 2 and GLRaV 3 using antibodies developed against their respective recombinant coat proteins.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Kai-Shu Ling

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Grapevine leafroll-associated virus 2