Journal of Phytopathology (2006) 154, 190-192

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A. Pérez-García, E. Mingorance, M.E. Rivera, D. Pino, D. Romero, J.A. Torés and A. Vicente (2006)
Long-term preservation of Podosphaera fusca using silica gel
Journal of Phytopathology 154 (3), 190-192
Abstract: Podosphaera fusca is the main causal agent of cucurbit powdery mildew in Spain and one of the most important limiting factors for cucurbit production worldwide. As an obligate biotrophic parasite, this fungus has been traditionally cultured and conserved by periodical transfers of conidia to fresh plant material. Here we describe a simple protocol for preservation of P. fusca isolates in absence of living tissue based on the dry spore, slow-freezing technique, and demonstrate that storage of silica gel desiccated conidia at −80°C is an efficient method for the long-term preservation of the pathogen.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Alejandro Pérez-García

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Podosphaera fusca Spain (continental)