Journal of General Plant Pathology (2014) 80, 475-478

Toshimasa Shiraishi, Hiroshi Sakai, Kentaro Ikeda and Toshihiko Urushibara (2014)
A useful method for preparing microsclerotial inoculum of Verticillium dahliae
Journal of General Plant Pathology 80 (6), 475-478
Abstract: Various methods of inoculating plants with Verticillium dahliae have been described; however, so far, there are no effective preparations of its microsclerotia, a major source of infection under natural field conditions. In this study, we developed a simple and effective method to prepare microsclerotial inoculum of V. dahliae using a cellophane medium that effectively promoted microsclerotia formation. In addition, a sieving process allowed the preparation of inoculum exclusively composed of microsclerotia. Our novel method provides a reproducible and stable inoculum for disease induction by V. dahliae under conditions that approximate natural infections.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Kentaro Ikeda, Hiroshi Sakai

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Verticillium dahliae	Aralia (crop)	Japan