Journal of General Plant Pathology (2008) 74, 61-65

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Toshiyuki Usami, Miho Fukaya and Yoshimiki Amemiya (2008)
Electrophoretic karyotyping and mapping of pathotype-specific DNA sequences in Japanese isolates of Verticillium dahliae
Journal of General Plant Pathology 74 (1), 61-65
Abstract: The chromosome number and electrophoretic karyotype of Japanese isolates of Verticillium dahliae were investigated. In a genomic Southern blot analysis of seven isolates probed with a telomere consensus sequence (TTAGGG)5, 12 or 14 bands were observed. Furthermore, pulsed-field gel electrophoresis (PFGE) of these isolates revealed five or six chromosomal bands. A band (approx. 3.5 Mbp) common to all isolates apparently contained more than two chromosomes. From these results, we concluded that each isolate's chromosome number is six (an eggplant pathotype isolate) or seven (all isolates of tomato and sweet pepper pathotypes). Although the chromosome sizes differed among isolates, karyotypes were similar within tomato and sweet pepper pathotypes. A small chromosome (approx. 1.8 Mbp) was observed only in the sweet pepper pathotype. Subsequent PFGE-Southern hybridization analyses revealed that the three DNA fragments specific to tomato pathotype are located on the same chromosome. These results suggest that the tomato-pathotype-specific DNA sequences might coexist on one chromosome.
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Link to article at publishers website
Database assignments for author(s): Toshiyuki Usami

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Verticillium dahliae Green pepper/chilli (Capsicum) Japan
Verticillium dahliae Tomato (Solanum lycopersicum) Japan