Journal of Economic Entomology (1998) 91, 579-584

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D.A. Andow, D.N. Alstad, Y.-H. Pang, P.C. Bolin and W.D. Hutchison (1998)
Using an F2 screen to search for resistance alleles to Bacillus thuringiensis toxin in European corn borer (Lepidoptera: Crambidae)
Journal of Economic Entomology 91 (3), 579-584
Abstract: We present an application of an F2 screening method for recovering and estimating the frequencies of rare alleles that confer insect resistance to a transgenic corn variety producing Bacillus thuringiensis Berliner crystal protein toxin (Bt corn). Based on a sample of 91 female Ostrinia nubilalis (Hübner) we show with 95% confidence that the frequency of B. thuringiensis resistance alleles from a wild Minnesota population is <0.013. This is an upper limit to the estimated allele frequency and does not provide clear evidence that 1 of the assumptions of the refuge plus high-dose strategy will or will not be met. With additional sampling, a more precise estimate of resistance allele frequency could be obtained that would clearly support or refute 1 of the assumptions of the refuge plus high-dose strategy. Variable costs of the screening method were $19.70 per female line, but these could be reduced by improved collecting, rearing, and handling methods.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): William (Bill) D. Hutchison

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
resistance/tolerance/defence of host

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Ostrinia nubilalis U.S.A. (mid N)
Bacillus thuringiensis (entomopathogen) Ostrinia nubilalis