Journal of Economic Entomology (1994) 87, 1157-1164

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Mark A. Wolff, Yehia A.I. Abdel-Aal, Doreen K.S. Goh, Emmett P. Lampert and R. Michael Roe (1994)
Organophosphate resistance in the tobacco aphid (Homoptera Aphididae): Purification and characterization of a resistance-associated esterase
Journal of Economic Entomology 87 (5), 1157-1164
Abstract: A 1-naphthyl acetate esterase was identified by isoelectric focusing in organophosphate resistant (red) tobacco aphids, Myzus nicotianae Blackman, which apparently was lacking in susceptible (green) individuals of the same species. This resistance-associated esterase (RAE) was purified from the cytosol of whole body homogenates of adult tobacco aphids by gel permeation chromatography and two successive DEAE-Sephacel ion exchange columns. The enzyme eluted from the second DEAE column in 0.23 to 0.28 M NaCl and was ~95% pure as determined by high-performance liquid chromatography (HPLC) on a BIO-SIL TSK-125 column. Purified RAE had an apparent molecular weight of 67,300 as determined by gel permeation HPLC and 66,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Wide range isoelectric focusing (pH 3.5-9.5) on polyacrylamide gel plates indicated that the isoelectric point was 4.61. Rabbit antiserum to the green peach aphid, M. persicae (Sulzer), esterase (E4) specifically cross-reacted with the tobacco aphid RAE, and the two esterases had identical molecular weights and isoelectric points as determined by immunoblotting. RAE protein was not detectable in susceptible tobacco aphids.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): R. Michael Roe

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Myzus nicotianae