Insects (2017) 8 (1 - 32)

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Alina Avanesyan, Benjamin D. Jaffe and Christelle Guédot (2017)
Isolating spermathecae and determining mating status of Drosophila suzukii: A protocol for tissue dissection and its applications
Insects 8 (1 - 32)
Abstract: The spotted wing drosophila, Drosophila suzukii (Diptera: Drosophilidae), is an emerging invasive pest, which attacks a wide variety of fruits and berries. Although previous studies have focused on different aspects of D. suzukii reproductive biology, there are no protocols available for determining the mating status of D. suzukii females and drosophilids in general. In this study, a step-by-step protocol for tissue dissection, isolating spermathecae, and determining the mating status of females was developed specifically for D. suzukii. This protocol is an effective and relatively quick method for determining female mating status. It has important applications from exploring reproductive output of D. suzukii females to understanding the biology of D. suzukii winter morph, which presumably plays the main role in the overwintering of this invasive species. We demonstrated applicability of this protocol for both field collected flies and flies reared in the lab, including fly specimens stored on a long-term basis.
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Full text of article
Database assignments for author(s): Christelle Guédot, Alina Avanesyan

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Drosophila suzukii