Insect Science (2008) 15, 423-428

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Min-Juan Meng, Tian-Long Li, Chang-You Li and Guo-Xun Li (2008)
A suspended cell line from Trichoplusia ni (Lepidoptera): Characterization and expression of recombinant proteins
Insect Science 15 (5), 423-428
Abstract: A suspended cell line from Trichoplusia ni embryos was established, and its susceptibility to Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) infection was investigated. This cell line had characteristics distinct from the BTI-Tn5Bl-4 cell line (Tn5Bl-4) from T. ni in growth, and showed approximately the same responses to AcMNPV infection, production of occlusion bodies, and levels of recombinant protein expression. No clumps were observed at maximum cell density at late-log phase in shake-flask or T-flask cultures, and thus the cells represent a useful new contribution for baculovirus research. The cells consist of two major morphological types: approximately 70% spindle-shaped cells and 30% round cells. The cell line was highly susceptible to virus infection and produced around 107 AcMNPV occlusion bodies per cell, on average. Production of ß-galactosidase and secreted alkaline phosphatase was high with 3.97 ± 0.13 × 104IU/mL and 3.48 ± 0.40 IU/mL, respectively. This cell line may be applicable for studies of scale-up production of viruses or baculovirus-insect cell expression. We also believe the new line can be a source for cell clones with higher production of virus and recombinant proteins compared to the parent or other existing cell lines such as Tn5Bl-4.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website


Research topic(s) for pests/diseases/weeds:
rearing/culturing/mass production
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
rearing/culturing/mass production


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Trichoplusia ni
Autographa californica nucleopolyhedrovirus (entomopathogen) Trichoplusia ni