Fitopatologia Brasileira (2007) 32, 318-328

From Pestinfo-Wiki
Jump to: navigation, search

Luiz Carlos C. Almeida and Rildo S.B. Coêlho (2007)
Caracterização da agressividade de isolados de Colletotrichum de maracujá amarelo com marcadores bioquímico, fisiológico e molecular
[Characterization of aggressiveness of Colletotrichum isolates from yellow passion fruit by biochemical, physiological and molecular markers]
Fitopatologia Brasileira 32 (4), 318-328
Abstract: Anthracnose is the most important post harvest disease of yellow passion fruit, whose etiologic agent has been identified in Brazil as Colletotrichum gloeosporioides. For pathogen characterization, 33 isolates were obtained from three producing regions of Pernambuco State. Morphological characters, such as color of the colony, conidial form and dimension, perithecium production and specific primers for C. gloeosporioides, C. acutatum and "Colletotrichum of Passiflora" allowed the identification of Glomerella cingulata pathotype 1, G. cingulata pathotype 2, Colletotrichum sp. from Passiflora and Colletotrichum sp. from yellow passion fruit. Yellow passion fruit inoculations showed two isolate groups, one with high aggressiveness (GA-1) and another with low aggressiveness (GA-2). Biochemical markers, such as amilolytic, cellulolytic, lipolytic and proteolytic enzymatic activities and physiological markers, such as mycelial growth, did not separate isolates into groups by aggressiveness. Standard markers produced by DNA amplification of isolates, using RAPD primers, showed that GA-1 isolates were more genetically related to each other than GA-2 isolates, evidencing GA-1 isolates with more recent evolution. The DNA amplifications of isolates with OPA-9 primer generated a marker that allowed grouping of 85.7% of GA-1 isolates, but included some GA-2 isolates with aggressiveness close to that of GA-1 isolates; therefore, OPA-9 primer can be used to characterize Colletotrichum spp. isolates with high aggressiveness in genetic resistance programs.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
Full text of article

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Colletotrichum gloeosporioides Passionfruit (Passiflora) Brazil (NE)