Fitopatologia Brasileira (2004) 29, 441-446

Laura Otero, Daniel Ducasse and Robert N.G. Miller (2004)
Variability in ribosomal DNA genic and spacer regions in Verticillium dahliae isolates from different hosts
Fitopatologia Brasileira 29 (4), 441-446
Abstract: Using PCR-based assays with specific primers for amplification of the ribosomal DNA intergenic spacer region (IGS) and a portion of the mitochondrial DNA small subunit ribosomal RNA gene (mtDNA SSU rRNA), the genetic variability among Verticillium dahliae isolates from olive (Olea europaea) and other host species from Argentina and Brazil was estimated. The derived UPGMA-generated phenograms based upon the restriction fingerprinting data of rDNA IGS products revealed genetic differences, correlating with the host of origin. Isolates infecting olive genetically distinct from those from cocoa (Theobroma cacao) and sunflower (Helianthus annuus). Digestion of mitochondrial DNA SSU rRNA PCR products revealed less variability, distinguishing only one isolate from sunflower. Ribosomal DNA ITS restriction patterns were identical for all isolates of V. dahliae, irrespective of host of origin. These preliminary results may have relevance for Verticillium wilt control practices, possibly reflecting a different evolutionary origin, or reproductive isolation of the pathogen in olive, distinct from populations of other hosts.
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Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Verticillium dahliae	Sunflower (Helianthus annuus)	Argentina
	Verticillium dahliae	Olive (Olea europaea)	Argentina
	Verticillium dahliae	Cacao (Theobroma cacao)	Brazil (NE)