FEMS Microbiology Letters (2004) 233, 69-76

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Anthony Wilson, Duncan Simpson, Elizabeth Chandler, Phil Jennings and Paul Nicholson (2004)
Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae
FEMS Microbiology Letters 233 (1), 69-76
Abstract: Isolates of the type-A trichothecene producing Fusarium sporotrichioides and Fusarium langsethiae were grouped and differentiated in a phylogenetic tree using ITS sequence dissimilarity. An attempt was made to develop a PCR-based assay for the detection and differentiation of Fusarium sporotrichiodes from other Fusarium species using the 5'-region of the tri5 gene as a template. However, this assay was unable to differentiate, to a satisfactory level, between isolates of Fusarium sporotrichioides and Fusarium langsethiae, providing further genetic evidence for their close genetic relationship. A robust and repeatable PCR-assay was developed for the detection and differentiation of both species based on sequence determined from differentially amplified RAPD-PCR products. These assays were able to detect both species in samples of grain taken from the field.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Paul Nicholson

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Fusarium sporotrichioides
Fusarium langsethiae