European Journal of Plant Pathology (2014) 139, 241-244

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André Gomes Coelho Souza, Sonia Herrero, Luiz Antonio Maffia and Margaret Elizabeth Daub (2014)
Methods for Cercospora coffeicola protoplast isolation and genetic transformation with the green fluorescent protein
European Journal of Plant Pathology 139 (2), 241-244
Abstract: Cercospora coffeicola is the causal agent of brown eye spot on coffee leaves. Although the disease has significant importance, few molecular studies have been done with C. coffeicola. Here we report a protocol for isolating protoplasts as well as development of a genetic transformation system using Green Fluorescent Protein. High yields of protoplasts (≈108/ml) were obtained from mycelial cultures from five isolates of C. coffeicola. One isolate was transformed with a vector encoding hygromycin resistance and Green Fluorescent Protein. Out of 43 hygromycin-resistant transformants obtained, Green Fluorescent Protein was highly expressed in one (2.3 %).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Luiz A. Maffia

Research topic(s) for pests/diseases/weeds:
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Cercospora coffeicola Coffee (Coffea) Brazil (south)