Entomological Science (2012) 15, 121-126

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Wonhoon Lee and Seunghwan Lee (2012)
Unexpected problem in aphid DNA barcoding by universal primers
Entomological Science 15 (1), 121-126
Abstract: DNA barcode (mitochondrial COI) sequences have allowed for species identification of aphids. In this study, we newly found a DNA barcoding problem in a part of the DNA sequences for Sitobion avenae. Five S. avenae individuals showed differences of, on average, 32.60% in the DNA sequences from other conspecific individuals, and a BLAST search revealed that the five sequences are similar to those of aphid parasitoids such as Aphidius, Ephedrus and Praon spp. (Hymenoptera: Braconidae). Based on these results, we concluded that the universal primers used in aphid DNA barcodes can amplify barcode sequences from parasitoid species within host aphids.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Seung Hwan Lee

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Sitobion avenae Maize/corn (Zea mays) Korea-South
Sitobion avenae Rosa (crop) Korea-South
Sitobion avenae Agropyron (crop) Korea-South
Sitobion avenae Akebia (crop) Korea-South