Entomologia Sinica (2000) 7, 329-336

From Pestinfo-Wiki
Jump to: navigation, search

Yong-Dan Li and Li-Ying Wang (2000)
Ultrastructure and DNA restriction endonuclease analysis of Calliptamus italicus entomopoxvirus
Entomologia Sinica 7 (4), 329-336
Abstract: Calliptamus italicus entomopoxvirus (CiEPV) was isolated from the grasshopper Calliptamus italicus. This virus mainly infects the host's fat body, and its development in the cytoplasm includes 4 steps: the appearance of viroplasm in the cytoplasm of fat body; the formation of immature virions by budding from viro-plasm; the differentiation of mature virions from immature virions and the accumulation of occlusion-body protein. The mature virions are oval with mulberry-like surface, measuring 190 nm X 350 nm in size. Most of the occlusion bodies are elliptical, some are square- round in shape. There are great differences in size ranging from 1.5 µm X 1.9 µm to 8.8 µm X 12.3 µm. CiEPV DNA, when cleaved with restriction endonuclease EcoR I Bg1 II and Hin d III, produces 16-18 and 10 fragments respectively, and the molecular weight of this DNA was estimated to be 135.7 X 106 daltons. Some characteristics of morphology and DNA were compared between CiEPV and Oedaleus asiaticus EPV (OaEPV) reported previously.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Calliptamus italicus
Calliptamus italicus entomopoxvirus (entomopathogen) Calliptamus italicus