Entomologia Experimentalis et Applicata (2014) 153, 85-92

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Feng Liu, Xiuzhen Shi, Yanpo Liang, Qingjun Wu, Baoyun Xu, Wen Xie, Shaoli Wang, Youjun Zhang and Nannan Liu (2014)
A 36-bp deletion in the alpha subunit of glutamate-gated chloride channel contributes to abamectin resistance in Plutella xylostella
Entomologia Experimentalis et Applicata 153 (2), 85-92
Abstract: Diamondback moth (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae), is one of the most destructive pests in Brassicaceae crops, such as Chinese cabbage (Brassica rapa L.). It is rapidly developing resistance to abamectin, the dominant insecticide utilized in controlling P. xylostella in China and other southeastern Asian countries. The target of abamectin, the alpha subunit of glutamate-gated chloride channel (GluClalpha), is thought to be involved in the development of abamectin resistance in nematodes and insects. This study investigated variants of GluClalpha in both abamectin-susceptible and resistant strains of P. xylostella. A comparison of the PxGluClalpha sequences revealed three variants, including a 63-bp substitution, a 36-bp deletion, and a 65-bp insertion. The frequency of the 36-bp deletion was much higher in the abamectin-resistant strain compared to the susceptible strain, whereas the 63-bp substitution and 65-bp insertion showed no significant difference between the resistant and susceptible strains. The in vitro expression of PxGluClalpha (with or without the 36-bp deletion) in Xenopus laevis (Daudin) oocytes indicated that PxGluClalpha with the 36-bp deletion was less sensitive to both glutamate and abamectin compared to the wild-type PxGluClalpha. These findings suggest that the variant 36-bp deletion in PxGluClalpha may confer abamectin resistance in P. xylostella after continuous abamectin selection, providing new insights into the management of this pest and contributing to the development of new reagents for pest control.
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Link to article at publishers website
Database assignments for author(s): Qing Jun Wu, Shaoli Wang, Nannan Liu

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Plutella xylostella