Entomologia Experimentalis et Applicata (1996) 79, 25-32

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E.W. Davidson, R.B.R. Patron, L.A. Lacey, R. Frutos, A. Vey and D.L. Hendrix (1996)
Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system
Entomologia Experimentalis et Applicata 79 (1), 25-32
Abstract: An assay system was developed for the adult silverleaf whitefly, Bemisia argentifolii Bellows and Perring (Homoptera: Aleyrodidae). This practical device was constructed from standard disposable laboratory materials. Whiteflies were harvested directly from the leaf and into a collection vial by vacuum aspiration, minimizing physical damage to the insect. Insects were fed through a cellulose mixed-ester membrane on a diet of 20-27% sucrose alone or sucrose in an extract of zucchini (Curcurbita moschata Duchense). Mortality and honeydew production were scored. At 22-25 °C and 50-55% relative humidity, control mortality generally remained at or below 15% during a 48 h assay period. The bioassay system was first tested using the insecticide, Imidacloprid, then used to screen a number of natural products with potential insecticidal activity against the whitefly. Destruxins extracted from the entomopathogenic fungus, Metarhizium anisopliae, and the natural insecticide/nematicide, Ivermectin, as well as bee venom and two of its components, melittin and phospholipase A2, were found to be toxic to B. argentifolii. Five lectins, Bacillus thuringiensis toxins, gossypol, an extract of Paeciliomyces fumosoroseus, wasp and scorpion venom, and a trypsin inhibitor were not found to be insecticidal to adult B. argentifolii.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Lawrence A. Lacey

Research topic(s) for pests/diseases/weeds:
control - general


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Bemisia tabaci biotype MEAM1