EPPO Bulletin (2002) 32, 467-469

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B.P. Borowicz, A. Mackowiak and H. Pospieszny (2002)
Improved identification of Pseudomonas savastanoi pv. phaseolicola at the molecular level
EPPO Bulletin 32 (3), 467-469
Abstract: Polymerase chain reaction (PCR) technology was used to identify Pseudomonas savastanoi pv. phaseolicola at the DNA level. Oligonucleotide primers were designed on the basis of the DNA sequence of the phaseolotoxin gene cluster of P. s. phaseolicola. Identification of the pathogen was significantly improved and appearance of unspecific bands was greatly diminished by experimentally selecting the most suitable annealing temperature (Tm) value. We obtained a single, strong DNA band of 1.4 kb length, specific for the identification of P. s. phaseolicola, using a PCR programme based on a Tm value of 80 °C.
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Link to article at publishers website
Database assignments for author(s): Henryk Pospieszny

Research topic(s) for pests/diseases/weeds:
identification/taxonomy


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Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Pseudomonas syringae pv. phaseolicola Beans (Phaseolus) Poland
Pseudomonas syringae