Bulletin of Insectology (2011) 64, S61-S62

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Gulnara Balakishiyeva, Madat Qurbanov, Alamdar Mammadov, Shahniyar Bayramov and Xavier Foissac (2011)
Detection of 'Candidatus Phytoplasma brasiliense' in a new geographic region and existence of two genetically distinct dnaK genotypes
Bulletin of Insectology 64 (Suppl.), S61-S62
Second International Phytoplasmologist Working Group Meeting (IPWG), Neustadt an der Weinstraße (Germany) September 12-15, 2011
Abstract: In September 2007, a peach tree (Prunus persica) displaying yellowing symptoms indicative of phytoplasma infection was sampled in Quba region of Azerbaijan. A phytoplasma was detected in the diseased peach tree by nested PCR amplification of its 16Sr DNA with universal primers for phytoplasmas. Phylogenetic analyses of the amplified 16S rDNA showed that the phytoplasma infecting peach tree corresponded to `Candidatus Phytoplasma brasiliense', a phytoplasma not previously reported in the Euro-Mediterranean area. To set up a detection assay, cloning of a `Ca. P. brasiliense' DNA fragment was undertaken by comparative RAPD. The amplified dnaK-dnaJ genetic locus was used to design a nested PCR assay able to amplify all `Ca. P. brasiliense' strains of the subgroup 16SrXV-A without amplifying the related members of the group 16SrII. The use of this assay also confirmed detection for the first time of `Ca. P. brasiliense' in diseased basil collected in south Lebanon.
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Database assignments for author(s): Xavier Foissac

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Phytoplasma brasiliense Peach/nectarine (Prunus persica) Azerbaijan
Phytoplasma brasiliense Basil (Ocimum) Lebanon