Bulletin of Insectology (2007) 60, 249-250

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J. Boben, N. Mehle and M. Ravnikar (2007)
Optimization of extraction procedure can improve phytoplasma diagnostics
Bulletin of Insectology 60 (2), 249-250
First International Phytoplasmologist Working Group Meeting (IPWG), Bologna (Italy) November 12-15, 2007
Abstract: Phytoplasma cause many important vector-borne and graft-transmissible plant diseases. They can affect different host plants. In this work we concentrated on the optimization of diagnostic procedures of pyhtoplasma on fruit trees. The extraction of DNA from the samples is necessary for the molecular analyses that follow. In our experiment we compared two extraction methods - CTAB and automatic extraction procedure on 61 different samples of fruit trees. After the analysis with real-time PCR we concluded that both extraction procedures give the same result, but the automated procedure is less time consuming and therefore has advantage over the CTAB method.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
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Database assignments for author(s): Jana Boben, Natasa Mehle

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surveys/sampling/distribution


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Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Phytoplasma pruni Slovenia
Phytoplasma mali Slovenia
Phytoplasma pyri Slovenia